Is there a way to associate a participant number with each submission?

Is there an option with jotform that gives a number to each form that is answered so that we may do a "test-retest"

To associate a unique participant number for each submission, I think the Unique ID Widget would fit your needs.

Complete guide: How-to-Add-Custom-Unique-IDs-to-your-Form-Submissions

The guide above will walk you through the entire setup so please give it a whirl and let us know if you see any issues.