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Yes you can and you have 2 options to choose from:
1. Use a TEXT FIELD that will contain the line you want to add. You will use the <hr> tag to achieve that.
2. Or you can use a widget:
a. Basic Separator: https://widgets.jotform.com/widget/separator
b. Form Separators: https://widgets.jotform.com/widget/form_separators
Here's a guide on How-to-Add-a-Widget-to-your-Form
Hope this answered your question.