Microtomy Knowledge Assessment
Assess your understanding of microtomy principles, techniques, and best practices.
Full Name
*
First Name
Last Name
Email Address
*
example@example.com
Current role or position
*
Please Select
Histotechnologist
Laboratory Technician
Pathology Assistant
Student
Other
How many years of experience do you have with microtomy?
*
Please Select
Less than 1 year
1-3 years
4-7 years
8+ years
Which of the following is the primary purpose of using a microtome?
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To cut thin sections of tissue for microscopic examination
To stain tissue samples
To fix tissues
To dry tissue slides
Match the microtomy terms with their correct definitions.
*
Rows
Definition
Microtome chuck
A device for holding and advancing tissue blocks
A blade used for sectioning
A solution used to float sections
A process of trimming excess paraffin
Microtome knife
A device for holding and advancing tissue blocks
A blade used for sectioning
A solution used to float sections
A process of trimming excess paraffin
Water bath
A device for holding and advancing tissue blocks
A blade used for sectioning
A solution used to float sections
A process of trimming excess paraffin
Facing
A device for holding and advancing tissue blocks
A blade used for sectioning
A solution used to float sections
A process of trimming excess paraffin
Rate your confidence in performing the following microtomy tasks:
*
Rows
Not confident
Somewhat confident
Confident
Very confident
Trimming paraffin blocks
1
2
3
4
Cutting ribbons of paraffin sections
5
6
7
8
Mounting sections on slides
9
10
11
12
Changing microtome blades safely
13
14
15
16
Which of the following are common causes of section compression or wrinkling? (Select all that apply)
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Dull blade
Too fast cutting speed
Incorrect block temperature
Insufficient dehydration
Other
Which safety precautions should always be observed when using a microtome?
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Always use blade guards when not cutting
Wear appropriate personal protective equipment (PPE)
Dispose of blades in a sharps container
Leave the microtome unlocked when unattended
Other
What is the ideal thickness for most paraffin-embedded tissue sections?
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3-5 micrometers
10-15 micrometers
20-25 micrometers
1-2 micrometers
Describe a troubleshooting step you would take if your sections are consistently folding or wrinkling.
Please provide any additional comments or feedback regarding this assessment or your microtomy experience.
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